Construction of plasmid-based expression vector for Bacillus subtilis

Construction of plasmid-based expression vector for Bacillus subtilis

EnglishPaperback / softbackPrint on demand
Nguyen, Hoang D.
Scholar's Press
EAN: 9786202300728
Print on demand
Delivery on Friday, 28. of February 2025
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Detailed information

Plasmids are useful tools to study gene expression and their function. However, most of the available plasmids for Bacillus subtilis suffer from structural instability because of their rolling-circle replication mechanism. In this work, stable plasmids have been constructed allowing expression of recombinant proteins in the cytoplasm and their secretion into the culture supernatant. The author has also established an experimental system to immobilize proteins on the cell wall of B. subtilis. The sorting of surface proteins to the cell wall in B. subtilis has been investigated aiming to anchor recombinant protein on the surface.
EAN 9786202300728
ISBN 6202300728
Binding Paperback / softback
Publisher Scholar's Press
Pages 136
Language English
Dimensions 220 x 150
Authors Nguyen, Hoang D.